商品介紹

NATIVEN 製備級電泳系統

產品型號: AE-6760

特點:

• 高解析度的蛋白質分離與蒐集
• 氣吹式蒐集系統, 降低sample稀釋與汙染風險
• 針對大量之sample, 可選用漏斗型管柱
• 依sample volume 提供 S or L 兩型
• Concentrate & recover samples without loss
• easy - to - Use
• Excellent reproducibility

 

應用: 

  ◇ Fractionation of proteins / DNA using SDS-PAGE 

  ◇ Fractionation of proteins from Native-PAGE keeping with their activites 

  ◇ Concentration of the body fluids (ex. cell-extract, cell culture medium, serum, urine)

  ◇ Purification of antibody epitope 

  ◇ Preparation for Mass Spectrometry 

  ◇ Screening  

 

原理: 

ATIVEN® carries out the preparative electrophoresis by the repeating of 4 processes; separating, pausing, collect-ing and refilling. 

NATIVEN® gel column is made by polyacrylamide disc-gel. It’s separated 3 parts; upper gel, collection part and lower gel. The collection part is filled with the collection buffer. 

The samples is applied to the upper gel and separated by electrophoresis. In the separating phase, the protein bands are eluted into the collection part. Then, the elec-trophoresis paused (pausing phase). Keeping the pause, eluted bands are pushed by air to fraction collector (collecting phase). After the collecting, the collection part is sequentially refilled with a new collection buffer.

 

操作流程: 

 

	1. Sample Preparation Samples are available to have been completely solved in sample buffer of PAGE It’s recommended that you pre-purify your samples removing inhibitors of electrophoresis
	2. Test PAGE of samples It’s recommended very highly that you check your samples using slab-gel electrophoresis (Test PAGE) The data from Test PAGE are available to set the NATIVEN® program
	3. Assembly of NATIVEN® At first, you have to assemble the NATIVEN® gel column Next, you should assemble the NATIVEN® main body, accessories and options
	4. Select & Input programs to set experimental condition 4 parameters need to input in the NATIVEN® program These parameters highly affect the outcome of preparative electrophoresis
	5. Preparative electrophoresis using NATIVEN® After NATIVEN® running, the preparative electrophoresis processes automatically It’s possible to pause & readjust the program if you have trouble in preparative electrophoresis

 

 

案例說明:

 

A. High resolution protein separation

 

 

 

 

B. Concentrate & recover samples without loss

 

In preparative electrophoresis of NATIVEN®, it’s available to concentrate the focused proteins from the samples of weak concentration. The left figure shows the concentration of same proteins including bovine serum.     bands are more concentrated than the before-separation (bovine serum).

 

 

C. Detection of enzyme activities following SDS-PAGE separation

 

 

SDS-PAGE separation has good resolution of samples, but the isolated proteins activity are lost after that.  Some proteins, not of all, are possible to recover the activities by SDS removing after SDS-PAGE separation.  Following data shows the re-covery of enzyme activities following SDS-PAGE separa-tion using lysozyme.

 

 

 

案例統計: 

 

Various size protein or DNA can be separated with SDS/Native electrophoresis by NATIVEN®. 

26 out of 36 experiments are successful in collecting protein samples with their activities conserved.

 

 

規格: